Panel 3 (ASH)

3. Replacement vector for targeted disruption of the murine HCII gene
The blue boxes represent exons 1-4 of the HCII gene. The thick lines represent genomic DNA; the thin lines, vector DNA. The neomycin phosphotransferase gene (neo) was inserted in place of ~1 kb of 5' flanking sequence, exon 1, and part of intron 1.
The probe used for Southern blotting hybridizes with a sequence in exon 4. Digestion with SacI yields a 14-kb DNA fragment from the wild-type locus and a 6-kb fragment from the targeted locus.
RW-4 embryonic stem cells derived from 120/SvJ mice were transfected with the linearized targeting vector and selected for growth in the presence of G418.

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