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4. Disruption of the HCII gene in miceEmbryonic stem cells were injected into C57Bl/6 blastocysts and implanted into pseudopregnant females. The resulting chimeric males were crossed with wild-type C57Bl/6 females to produce F1 offspring. Heterozygous HCII+/- mice were crossed to produce HCII-deficient mice.
A. Southern blots of genomic DNA digested with SacI.
B. Western blots of plasma probed with goat anti-human HCII IgG (Affinity Biologicals, Hamilton, Ontario). The 68 kDa and 72 kDa bands represent the two glycoforms of HCII present in mouse plasma (Zhang et al., Biochemistry 33: 3632, 1994).