HCII activity in mouse plasma was determined by measuring the amount of human thrombin inhibited in the presence of dermatan sulfate.
Reaction mixtures (100 µl final volume) contained the following:
Mouse plasma (2 µl)After 1 minute, the amount of residual thrombin activity was determined by hydrolysis of a chromogenic substrate. The HCII activity is expressed as the mean + 1 SD (n = 9-11 animals of each genotype).
Dermatan sulfate (50 µg/ml)
Human thrombin (16 nM)
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